Breeding studies on red sea bream Pagrus major: mass selection to genome editing

Abstract Red sea bream Pagrus major is extremely important in the aquaculture of Japan. The breeding red bream, which has a history more than half century, can be divided into three main categories. first selective breeding. Aquaculture Research Institute, Kindai University, began fast-growing breams early 1960s through mass selection. I summarized results selection and characteristics fish 1990s. second chromosome manipulation sex control. Two types gynogenetic diploids (G2N) were artificially induced. Although growth survival G2N inferior, heterozygous clones produced by mating was comparable to normal diploids. differentiation process induction condition all-male groups 17α-methyltestosterone treatment clarified, percentage females could increased 85%. third genetic manipulation. We developed microinjection method, succeeded increasing edible ratio deleting function myostatin gene genome editing. Combining new technologies, such as editing, with existing methods mentioned above would result faster effective